Thebreast cancer has become a threat to women's lives at an increasing rate. Theinadequate treatments of surgical procedures and chemotherapy affect negativelyquality of patient life. Therefore, there has been an increase in demand foralternative and supportive agents, especially for the use of herbal products.The purpose of this study is to evaluate the in vitro anti-tumor activity of Lysimachia savranii in breast cancercell lines compared to normal adipose tissue derived mesenchymal stem cells. Theadipose tissue derived mesenchymal stem cells as normal cells with MCF-7 and,MDA-MB-231 breast cancer cells were cultured. IC50 doses of Lysimachia savranii extract for eachcells were determined via MTT assay. The immunocytochemical staining wasperformed for the oxidative stress by eNOS, whereas apoptosis were analyzed byTUNEL method. The data exhibited with H-score and apoptotic index. The plantextract inhibited the proliferation and survival in the breast cancer cells comparedto the stem cells. Lysimachia savraniiextract caused the increase of eNOS staining for oxidative stress and the TUNELlabeling for apoptosis. The further moleculartechniques should be used to determine the biologic activity of Lysimachiasavranii extract and its contentshould be investigated.