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Effect of the crude saponin extract from gypsophila pilulifera boiss. & heldr. On protease from bacillus subtilis atcc 6633 and antioxidant properties of the extract

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dc.contributor.author Yazıcı, Sercan Özbek
dc.contributor.author Özmen, İsmail
dc.date.accessioned 2019-02-28T13:53:58Z
dc.date.available 2019-02-28T13:53:58Z
dc.date.issued 2018 en_US
dc.identifier.citation Yazici, S. Ö., Özmen, İ. (2018) Effect of the crude saponin extract from gypsophila pilulifera boiss. & heldr. On protease from bacillus subtilis atcc 6633 and antioxidant properties of the extract. Iran J Sci Technol Trans Sci. 42: 1707. https://doi.org/10.1007/s40995-017-0366-y en_US
dc.identifier.uri http://hdl.handle.net/11672/991 en_US
dc.description.abstract The study was carried out to purify protease produced by Bacillus subtilis ATCC 6633 strain and to examine the effect of the crude saponin extract (CSE) obtained from Gypsophila pilulifera Boiss. & Heldr. on the purified enzyme. In addition, the present study dealt with the evaluation of antioxidant and DNA cleavage potentials of the extract. Protease from B. subtilis ATCC 6633 was produced and purified using ammonium sulfate precipitation, gel filtration chromatography with 27.2-fold. The purified enzyme showed a single band on SDS-PAGE, and it was determined that its molecular weight was 30.1 kDa. In addition, to examine the effect of saponin as natural surfactant on the protease activity, crude saponin from G. pilulifera was extracted in methanol and the purified protease was incubated with the extract. It was observed that the activity of enzyme increased in the presence of extract. In the in vitro assays, while total phenolic content of the extract was determined by Folin–Ciocalteau method, antioxidant properties were evaluated by DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical scavenging activity, FRAP (ferric-ion reducing antioxidant parameter), CUPRAC (cupric ion reducing antioxidant capacity) and β-caroten-linoleic acid method. The results obtained in this study showed that the extract possesses low antioxidant activity for studied all in vitro models. Also, effect of the extract on plasmid DNA was determined with agarose gel electrophoresis. The extract caused conversion of supercoiled DNA structure to other forms. As a result, the extract exhibited DNA cleavage activity as dose-dependent. en_US
dc.language.iso eng en_US
dc.publisher Springer en_US
dc.relation.isversionof ttps://doi.org/10.1007/s40995-017-0366-y en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject Antioxidant Activity en_US
dc.subject DNA Cleavage Activity en_US
dc.subject Enzyme Purification en_US
dc.subject Gypsophila Pilulifera Boiss. & Heldr en_US
dc.title Effect of the crude saponin extract from gypsophila pilulifera boiss. & heldr. On protease from bacillus subtilis atcc 6633 and antioxidant properties of the extract en_US
dc.type article en_US
dc.relation.journal Iranian Journal of Science and Technology, Transactions A: Science en_US
dc.contributor.department Mehmet Akif Ersoy Üniversitesi, Sağlık Bilimleri Fakültesi en_US
dc.identifier.volume 42 en_US
dc.identifier.issue 4 en_US
dc.identifier.startpage 1707 en_US
dc.identifier.endpage 1713 en_US


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