Özet:
Subclinical endometritis (SE) is one cause of repeat breeder syndrome (RBS). When endometrial cells come into contact with antigens during
inflammatory events like endometritis, they act like innate immune system cells. Ovsynch-based therapies stimulate the innate immune system,
which is how they may be able to cure SE and then RBS. This study aimed to assess the effects of presynch-10 and ovsynch applications on mRNA
expressions of some endometrial Toll- and Nod-like receptors (TLRs and NLRs) in cows with RBS. A total of 40 repeat breeder cows were used in the
study. The presynch-10 and ovsynch protocols were used in group 1 (G1, n=20), and no protocol was used in group 2 (G2, n=20). Endometrial samples
were collected before and after the synchronization protocols in G1. In G2, samples were collected, and after waiting time up to the duration of
synchronization protocol in G1, samples were taken again. The expression profiles of the genes being investigated were examined using RT-qPCR.
NLRC4, NALP3 and TLR4 were basically expressed in all of the samples while NOD2 expression was completely undetectable. There was a statistical
difference when NOD1 and TLR2 expressions in the second samples were compared between G1 and G2 (P<0.05). It was concluded that presynch-10
and ovsynch administration in cows may have a positively effect on the innate immune response and so be effective in the treatment of SE.
