Abstract:
The objectives of this study were to evaluate clinicopathological, immunohistochemical and electron microscopical findings of
Enzootic Nasal Adenocarcinoma (ENA) in five goats and examine the immunoreactivity of these tumors with different markers. At the
histopathological examination, tubular, papillary and acinar subtypes were diagnosed in this study. Tumor interstitial cells strongly
expressed S100, Neuron Specific Enolase (NSE), Smooth Muscle Actin (SMA) and Vimentin, epithelial cells were positive for
Pancytokeratin (PCK), Carcinoembryonic Antigen (CEA) and Gross Cystic Disease Fluid Protein (GCDFP). Both interstitial and epithelial
cells strongly expressed Caspase-3, Ki-67 and proliferating cell nuclear antigen (PCNA); both interstitial and epithelial cells were
negative for Alpha Fetoprotein (AFP). Immunohistochemical observations revealed that ENA consisted of both epithelial and
mesenchymal cell proliferations based on the increased PCNA and Ki-67 positive reaction of these cells. Electron microscopical studies
revealed the presence of the retrovirus-like particles in the cytoplasm of neoplastic epithelial cells. At the same time, this study is the
first report of ENA cases from Turkey in goats.
Description:
Bu çalışmanın amacı, 5 keçide Enzootic Nazal Adenokarsinomun klinik, patolojik, immunohistokimyasal ve elektron makroskobik
olarak incelenmesi ve bu tümörlerin değişik markırlar ile immunoreaktivitesinin incelenmesidir. Bu çalışmada tümörlerin histopatolojik
incelemesinde tubuler, papiller ve asiner subtipler teşhis edildi. Tümör interstiyel hücreleri S100, NSE, SMA ve Vimentin ile epitel
hücreleri ise PCK, CEA ve GCDFP ile pozitif reaksiyon verdi. Caspase-3, Ki-67 ve PCNA ile hem epitel hemde intertisyel hücrelerde pozitif
reaksiyon saptanırken, AFP bütün hücrelerde negatif reaksiyon gösterdi. Immunohistokimyasal incelemesi sonucunda ENA’da hem
epitel hemde intertisyel hücrelerde artış olduğu PCNA ve Ki-67 artışı ile saptandı. Elektron makroskobik incelemede neoplastik epitel
hücrelerinde retrovirus-benzeri yapılar saptandı. Bu çalışma aynı zamanda Türkiye’de keçilerde saptanan ilk ENA olgusudur.