This study was carried out in Kargı stream where located in Alanya district of Antalya province. Seven stations were choosen on the stream, Hydrachnidia (water mite) and water samples were taken from all stations between July 2014-April 2015 seasonally. Cl–, NH4+-N, NO2--N, NO3-N, PO4-P, biological oxygen demand of the water samples were analyzed in the laboratory. Water temperature, pH, dissolved oxygen, and electrical conductivity were measured during sampling in situ. As a result, a total of 32 taxa were detected, 9 taxa from Hygrobatidae, 8 taxa from Torrenticolidae, 6 taxa from Sperchontidae, 3 taxa from Lebertidae, 3 taxa from Hydryphantidae, 1 taxa from Aturidae, 1 taxa from Hydrodromidae and 1 taxa from Mideopsidae. Canonical Correspondence Analysis was applied to determine the relationships between the water mite fauna and physicochemical variables. Accordingly, pH, water temperature and biological oxygen demand were found as the most effective factors on distribution of water mite assemblages. This study represents the first effort for determining Kargı Stream of water mite fauna. So, all listed the species are firstly reported for the region.