Clinical, Pathological, Immunohistochemical and Ultrastructural Observations on Enzootic Nasal Adenocarcinoma in Five Goats

Abstract

The objectives of this study were to evaluate clinicopathological, immunohistochemical and electron microscopical findings of Enzootic Nasal Adenocarcinoma (ENA) in five goats and examine the immunoreactivity of these tumors with different markers. At the histopathological examination, tubular, papillary and acinar subtypes were diagnosed in this study. Tumor interstitial cells strongly expressed S100, Neuron Specific Enolase (NSE), Smooth Muscle Actin (SMA) and Vimentin, epithelial cells were positive for Pancytokeratin (PCK), Carcinoembryonic Antigen (CEA) and Gross Cystic Disease Fluid Protein (GCDFP). Both interstitial and epithelial cells strongly expressed Caspase-3, Ki-67 and proliferating cell nuclear antigen (PCNA); both interstitial and epithelial cells were negative for Alpha Fetoprotein (AFP). Immunohistochemical observations revealed that ENA consisted of both epithelial and mesenchymal cell proliferations based on the increased PCNA and Ki-67 positive reaction of these cells. Electron microscopical studies revealed the presence of the retrovirus-like particles in the cytoplasm of neoplastic epithelial cells. At the same time, this study is the first report of ENA cases from Turkey in goats.