Filamentöz faj üzerinde eksperese edilen Hepatit B yüzey antijenine karşı antikor yanıtı
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Mehmet Akif Ersoy University
Mehmet Akif Ersoy Üniversitesi
Mehmet Akif Ersoy Üniversitesi
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Fajlar organizmadahumoral ve hücresel immün yanıtıuyandırabilen yüksek immünojenik özelliğe sahip moleküllerdir ve potansiyel aşıtaşıyıcısı olarak kullanılmaktadırlar. Bu çalışmada Hepatit B yüzey antijeni (HBsAg)tam molekül olarak M13 faj yüzeyinde füzyon protein olarak ekprese edilmiş verekombinant fajın antijenik etkisi BALB/cJ farelerinde incelenmiştir. Farelerinyüksek dozda (1012) rekombinant faj ile immünize edilmesinin normalfaja kıyasla daha yüksek antikor yanıtına neden olduğu gösterilmiştir. BALB/cJfarelerinde elde edilen olumlu yanıt sonrası rekombinant fajın etkinliğiHepatit B virüsü (HBV) transgenik farelerdeve hibrid farelerde (C57BL/6JXBALB/c) incelenmiş ve ayrıca etkisi alüminyumadjuvantı ve mineral yağ adjuvantı gibi yaygın kullanımı olan adjuvantlarlakarşılaştırılmıştır. Çalışmanın sonuçları BALB/cJ farelerin, HBV transgenik farelerinve hibrid farelerin rekombinant faj ile immünizasyonunun antijene özgü antikoryanıtı oluşturduğunu göstermiştir. Sonuçlar ayrıca HBV taşıyıcılarında immünyanıt oluşturmak için güçlü adjuvantların kullanılması gerektiğini de işaretetmektedir.
Phages are highlyimmunogenic molecules capable of inducing both humoral and cellular immuneresponse and have been used as potential vaccine delivery vehicles. In thisstudy, the full-length Hepatitis B surface antigen (HBsAg) was expressed as HBsAg-pIIIfusion protein on M13 phage and the efficiacy of recombinant phage was studiedin BALB/cJ mice. It was shown that, immunization of mice with the highest doseof recombinant phage (1012) generated an increased antibody responsecompared to wild type phage immunization. After having a promising result inBALB/cJ mice, recombinant phage was tested in Hepatitis B virus (HBV)transgenic mice and hybrid mice (C57BL/6JXBALB/c) andits effect was also compared with the well known adjuvants such as alum andmineral oil adjuvants. Our results have shown that immunization of BALB/cJmice, HBV transgenic mice and hybrid mice with recombinant phage elicited an antigen-specificantibody response. The results also indicated that very strong adjuvants/carriersshould be used to induce immune response in HBV carriers.
Phages are highlyimmunogenic molecules capable of inducing both humoral and cellular immuneresponse and have been used as potential vaccine delivery vehicles. In thisstudy, the full-length Hepatitis B surface antigen (HBsAg) was expressed as HBsAg-pIIIfusion protein on M13 phage and the efficiacy of recombinant phage was studiedin BALB/cJ mice. It was shown that, immunization of mice with the highest doseof recombinant phage (1012) generated an increased antibody responsecompared to wild type phage immunization. After having a promising result inBALB/cJ mice, recombinant phage was tested in Hepatitis B virus (HBV)transgenic mice and hybrid mice (C57BL/6JXBALB/c) andits effect was also compared with the well known adjuvants such as alum andmineral oil adjuvants. Our results have shown that immunization of BALB/cJmice, HBV transgenic mice and hybrid mice with recombinant phage elicited an antigen-specificantibody response. The results also indicated that very strong adjuvants/carriersshould be used to induce immune response in HBV carriers.